个人简介
Dr. Zhixing Chen is a chemist with research experience spanning natural product synthesis, polymer mechano-chemistry, fluorescence and non-linear optical probes, bioconjugation, and live cell imaging. Zhixing believes in the enabling role of molecular technologies at the frontier of optical imaging, biotechnology, and life sciences. His current focus is to develop small and macro molecular tools for advanced imaging technologies. In the long term, Zhixing endeavors to unleash the synergy at the chemistry-biology-imaging interface in a collaborative manner, bringing new dimensions to the fields of molecular imaging and molecular medicine.
所授课程
Recent advance in bioimaging probes (3 credits)
Frontiers in Chemical Biotechnology and Bioimaging (3 credits)
General Chemistry (UG, 2 credits)
教育背景
Ph.D. 2009-2014 Chemistry Department of Chemistry, Columbia University
B.S. 2004-2008 Chemical Biology Department of Chemistry, Tsinghua University
工作经历
2018- now CLS Investigator, Peking-Tsinghua Center for Life Sciences, Peking University
Assistant Professor, Institute of Molecular Medicine, College of Future Technology, Peking University
2016-2018 Postdoctoral Researcher, Department of Chemistry, Stanford University (Advisor: Professor Yan Xia)
2015 Postdoctoral Researcher, Department of Chemistry, Columbia University (Advisor: Professor Wei Min)
2008-2009 Research Assistant, College of Chemistry and Molecular Engineering, PKU (Advisors: Professors Zhen Yang and Jiahua Chen)
承担项目
Beijing Municipal Science & Technology Commission
National Natural Science Foundation of China (General Program)
代表性论文
1. Zhe Yang, Yu Zhang, Yuxin Fang, Yuan Zhang, Jiasheng Du, Xiaowen Shen, Kecheng Zhang, Peng Zou* & Zhixing Chen*. Spatial barcoding reveals reaction radii and contact-dependent mechanism of proximity labeling. Nature Chemical Biology 2025, online
2. Dilizhatai Saimi, Luc Reymond, Tursunjan Aziz, Xuan Shen, Ziying Luo, Shuaibo Pi, Yitong Liu, Song Fu, Shuangjin Ding, Anming Meng, Liangyi Chen, Hui Jiang & Zhixing Chen*. Imaging mitochondrial membrane potential via concentration-dependent fluorescence lifetime changes. Nature Communications 2025, 16, 11088
3. Jing Ling#, Yuan Zhang#*, Yongzhen Hei#, Julian Kompa, Chen Yang, Bo Wang, Junwei Zhang, Jiasheng Du, Tatjana Rudi, Kecheng Zhang, Jingfu Sun, Wenjuan Wang, Sebastian Fabritz, Yulong Li, Wulan Deng, Peng Zou, Chunlai Chen*, Zhixing Chen*. Thioether editing generally increases thephotostability of rhodamine dyes on self-labeling tags, PNAS 2025, 122, e2426354122
4. Jing Ling#, Yitong Liu#, Alexandre Dumoulin#, Danli Sheng, Yunzhe Fu, Shuzhang Liu, Ling Ding, Lulu Huang, Peng Xi, Hongyun Tang, Esther T. Stoeckli, and Zhixing Chen; A gentle palette of plasma membrane dyes. PNAS 2025, 122, e2504879122
5. Junwei Zhang, Kecheng Zhang, Kui Wang, Bo Wang, Siyan Zhu, Hongping Qian, Yumiao Ma, Mengling Zhang, Tianyan Liu, Peng Chen, Yuan Shen, Yunzhe Fu, Shilin Fang, Xinxin Zhang, Peng Zou, Wulan Deng, Yu Mu, Zhixing Chen*. A Palette of Bridged Bicycle-Strengthened Fluorophores. Nature Methods 2025, 22，1276
6. Liu, T.#; Kompa, J.#; Ling, J.#; Lardon, N.; Zhang, Y.; Chen, J.; Reymond, L.; Chen, P.; Tran, M.; Yang, Z.; Zhang, H.; Liu, Y.; Pitsch, S.; Zou, P.; Wang, L.; Johnsson, K.; Chen, Z.* Gentle rhodamines for live-cell fluorescence microscopy. ACS Central Science 2024, 10, 1933-44.
7. Chen, J.; Stephan, T.; Gaedke, F.; Liu, T.; Li, Y. Schauss, A.; Chen, P.; Wulff, V.; Jakobs, S.; Jüngst, C.*; Chen, Z.* An aldehyde-crosslinking mitochondrial probe for STED imaging in fixed cells. PNAS 2024, 121, e2317703121.
8. Liu, S.#; Ling, J.#; et al.; Zou, P.*; Chen, Z.* Orange/far-red hybrid voltage indicators with reduced phototoxicity enable reliable long-term imaging in neurons and cardiomyocytes. PNAS 2023, 120, e2306950120.
9. Liu, T.#; Stephan, T.#; Chen, P.; Keller-Findeisen, J.; Chen, J.; Riedel, D.; Yang, Z.; Jakobs, S.; Chen, Z.* Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain. PNAS 2022, 119, e2215799119 [Cover]
10. Mo, J.#; Chen, J.#; Shi, Y.; Sun, J.; Wu, Y.; Liu, T.; Zhang, J.; Zheng, Y.; Li, Y.; Chen, Z.* Third-Generation Covalent TMP-Tag for Fast Labeling and Multiplexed Imaging of Cellular Proteins. Angew. Chem. Int. Ed. 2022, 61, e202207905
11. Zhang, J.#; Peng, X.#; Wu, Y.#; Ren, H.#; Sun, J.; Tong, S.; Liu, T.; Zhao, Y.; Wang, S.; Tang, C.; Chen, L.; Chen, Z.* Red- and Far-Red-Emitting Zinc Probes with Minimal Phototoxicity for Multiplexed Recording of Orchestrated Insulin Secretion. Angew. Chem. Int. Ed. 2021, 60, 25846-55. [Inside Cover]
12. Yang, Z.#; Li, L.#; Ling, J.; Liu, T.; Huang, X.; Ying, Y.; Zhao, Y.; Zhao, Y.; Lei, K.*; Chen, L.*; Chen, Z.* Cyclooctatetraene-conjugated cyanine mitochondrial probes minimize phototoxicity in fluorescence and nanoscopic imaging. Chemical Science 2020, 11, 8506-8516.
