摘要：
Super-resolution fluorescence microscopy, also known as fluorescence nanoscopy, represented a breakthrough for bioimaging as it delivers sub-diffraction resolution using far-field microscopes. Although they do not face any fundamental limit, the resolution of the first generation of methods was bound by the limited photostability of fluorophores under ambient conditions to about 10-30 nm resolution. This has motivated the development of a second generation of fluorescence nanoscopy aiming to surpass sub-10 nm resolutions, thus providing true biomolecular resolution. In this talk, I will present the latest efforts of our lab to address this challenge trough five different approaches: pulsed-interleaved MINFLUX, SIMPLER, STED-FRET, RASTMIN, and RASTED. In addition, the outstanding potential of MINFLUX for single-molecule/particle tracking will be discussed.
报告人简介：
Prof. Stefani holds a Ph.D. from the University of Mainz/Max Planck Institute for Polymer Research, was researcher at the Institute of Photonic Sciences (ICFO, Barcelona), Assistant Prof. at the LMU Munich, Max Planck Partner Group leader with Stefan W. Hell, co-developer of MINFLUX. Currently is Full Prof. at the Physics Dept. of the University of Buenos Aires, Superior Investigator of National Scientific and Technical Research Council and Director of the Center for Bionanoscience Research.
